Phytochemical Screening and Evaluation of the Antioxidant and Antiplasmodial Activities of "<i>Ahoutou</i>", a Recipe Used in the Treatment of Malaria in Ivory Coast

Gouegoui Serge-Pacome Bohui; Sylvain Beourou; Djibiliour Sanogo; Jean David N’guessan; Koffi Barthelemy Attioua; Augustin Amissa Adima

doi:doi:10.11648/j.sjc.20241205.14

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Phytochemical Screening and Evaluation of the Antioxidant and Antiplasmodial Activities of "Ahoutou", a Recipe Used in the Treatment of Malaria in Ivory Coast

Gouegoui Serge-Pacome Bohui^*

, Sylvain Beourou, Djibiliour Sanogo, Jean David N’guessan, Koffi Barthelemy Attioua, Augustin Amissa Adima

Published in Science Journal of Chemistry (Volume 12, Issue 5)

Received: 24 August 2024 Accepted: 12 September 2024 Published: 26 September 2024

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Abstract

Recipes based on medicinal plants are frequently used to treat malaria in Ivory Coast. Among these recipes is Ahoutou, made from a mixture of the leaves of three plants, one of the most widely used in the village of Djahakro in the commune of Yamoussoukro. However, the use of medicinal plant-based recipes in the fight against malaria must be based on scientific evidence of safety, efficacy and quality. The aim of this study is to provide a scientific, phytochemical and pharmacological basis for the traditional therapeutic use of Ahoutou. To this end, a phytochemical study of the Ahoutou extract was carried out, the antioxidant activity was assessed using the DPPH test and then the in vitro anti-plasmodial activity was carried out on clinical isolates and on the reference strain Dd2. The phytochemical study revealed the presence of polyphenols, flavonoids, alkaloids, tannins, saponins, steroids and triterpenes. The antioxidant activity test showed that Ahoutou was capable of reducing the DPPH free radical. Ahoutou showed promising anti-plasmodial activity (IC50 =12.86 µg/mL) in the clinical strain and moderate activity (IC50 =23.40 µg/mL) in the reference strain. Our results would justify the use of this recipe in the treatment of malaria. We plan to study the toxicity of Ahoutou.

Published in	Science Journal of Chemistry (Volume 12, Issue 5)
DOI	10.11648/j.sjc.20241205.14
Page(s)	109-116
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Ahoutou, Antioxidant, Antiplasmodial Activity

References

[1]	Rocha-Silva, F. L., Silva, A. C. P. D., Pohlit M. A., Quignard, J. L. E., Viera, R. P. P., Tadei, P. W., Chaves, M. C. F., Samonek, F. J., Lima, J. A. C., Costa, F. R. M., Alecrim, C. G. M. & Neto, A. F. V. (2011). In vivo and in vitro antimalarial activity of 4 nerolidylcatechol. Phytotherapy research 25, 1181- 1188. https://doi.org/10.1002/ptr.3424
[2]	WHO (2019). World malaria report 2019. World Health Organization, P. xxii.
[3]	Anonymous1 (2018). Malaria control in Côte d'Ivoire: deaths drop from 4431 in 2016 to 3222 in 2017. Press release of 12/04/2018, accessed on 25/06/2019.
[4]	Chenq, Q., Kyle, D. E. & Gatton, L. L. (2012). Artemisinin resistance in Plasmodium falciparum. A process linked to dormancy. International Journal for Parasitology Drugs and Drug Resistance 2, 249-255. https://doi.org/10.1016/ j.ijpddr 2012.01.001.
[5]	Cui, L., Mharakurwa, S., Ndiaye, D., Rathod, P. K. & Rosenthal, P. J. (2015). Antimalarial drug resistance literature review and activities and finding of the ICEMR network. American Journal of Tropical Medicine and Hygiene 93, 57-68. https://doi.org/10.4269/ajtmh.15-0007
[6]	Nondo, R. S., P. Erasto P., Moshi, M. J., Zacharia, A., Masimba, P. J. & Kidukuli, A. W. (2017). In vivo antimalarial activity of extracts of Tanzanian medicinal plants used for the treatment of malaria. Journal of Advanced Pharmacology and Technology Research 7, 59-63. https://doi.org/10.4103/2231-4040.179748
[7]	Adjanhoun, E. J., Adjakidje, V. & Ahyi, M. (1989). Médecine traditionnelle et pharmacopée: Contribution aux études ethnobotaniques et floristiques en république populaire du Bénin. Édrapport- ACCT Paris, 427p.
[8]	Dohou N, Yamni K, Gmira N, Idrissi Hassani LM. Study of polyphenols in the leaves of an Ibero-Moroccan endemic, Thymelaealychroides. Acta Bot Malacit.2003; 29: 233-239.
[9]	Wood, J. E., Senthilmohan, S. T. & Peskin, A. V. (2002). Antioxidant activity of procyanidin- containing plant extracts at different pHs. Food Chemistry 77, 155-161. https://doi.org/10.1016/S0308-8146(01)00329-6
[10]	Marinova, D., Ribarova, F. & Atanassova, M. (2005). Total phenolics in bulgarian Fruits and vegetables. Journal of the University of Chemical Technology and Metallurgy 40, 255-260. https://journal.uctm.edu/node/j2005-3/Marinova.pdf
[11]	Parejo I, Codina C, Petraski C, Kefalas P. (2000). Evaluation of scavenging activity assessed by co (11)/EDTA-induced luminal chemilunescence and DDPH (2, 2-diphenyl-1 -pycrilhydrazyl) free radical assay. J. Pharmacol. Toxicol. Method 44: 507-512.
[12]	Trager, W. & Jensen, J. B. (1976). Human malaria parasites in continuous culture. Science 190, 673-675. https://doi.org/10.1126/science.781840
[13]	Le-Nagard, H., Vincent, C., Mentre, F. & Bras, J. L. (2011). Online analysis of in vitro resistance to antimalarial drugs through nonlinear regression. Computer Methods and Programs in Biomedicine 104, 10-18. https://doi.org/10.1016/j.cmpb. 2010.08.003
[14]	Abalaka, M., Oyewole, O. A. & Kolawole, A. R. (2012). Antibacterial Activities of Azadirachta Indica against Some Bacterial Pathogens. Advances in Life Sciences 2, 5-8. https://doi.org/10.5923/j.als.20120202.02
[15]	Figueirinha, A., Paranhos, A., Pérez-Alonso, J. J., Santos-Buelga, C., Batista, M. T. & Kolawole, A. R. (2008). Cymbopogon citratus leaves: characterisation of flavonoids by HPLC-PDA-ESI/MS/MS and an approach to their potential as a source of bioactive polyphenols. Food Chemistry 110, 718-728. https://doi.org/10.1016/J.FOODCHEM.2008.02.045
[16]	Biswas, B., Rogers, K., McLaughlin, F., Daniels, D. & Yadav, A. (2013a). Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria. International Journal of Microbiology 2013, 7. https://doi.org/10.1155/2013/746165
[17]	Hsu, C. Y., Chan, Y. P. & Chang, J. (2007). Antioxidant activity of extract from Polygomum cuspidatum. Biol Res 40, 13-21. https://doi.org/10.4067/s0716-97602007000100002
[18]	Miguel, M. G., Cruz, C., Faleiro, L., Simoes, M. F. F., Figueiredo, A. C., Barroso, J. G. & Pedro, L. G. (2010). Foeniculum vulgare: chemical composition, antioxidant and antimicrobial activities. Natural Product Communications 5, 319-328. https://doi.org/10.1080/14786419.2010.499513
[19]	Zeng, W. & Wang, S. Y. (2001). Antioxidant activity and phenolic compounds in selected herbs. Journal of Agriculture and Food Chemistry 49, 5165-5170. https://doi.org/10.1021/jf010697n
[20]	Chen, I., Clarke, S. E., Gosling, R., Hamainza, B., Killeen, G., Magill, A., O'Meara, W., Price, R. N. P. & Riley, E. M. (2016). Asymptomatic malaria: a chronic and debilitating infection that should be treated. PLoS Medicine 13, 100-1942.
[21]	Meir, S., Kanner, J., Akiri, B. & Hadar, S. P. (1995). Determination and involment of aqueous reducing compounds in oxidative systems of various senescing leaves. Journal of Agriculture and food chemistry 43, 1813-1819. https://dx.doi.org/10.1021/jf00055a012
[22]	Han, X., hen, T. & Lou, H. (2007). Dietary Polyphenols and Their Biological Significance. International Journal of Molecular Sciences 8, 950-988. https://doi.org/10.3390/i8090950
[23]	Bacon, D. J., Latour, C., Lucas, C., Colina, O., Ringwald, P. & Picot, S. (2009). Comparison of a SYBR green I-based assay with a histidine-rich protein II enzyme-linked immunosorbent assay for in vitro antimalarial drug efficacy testing and application to clinical isolates. The Antimicrobial Agents and Chemotherapy 51, 1172-1178.
[24]	Beourou, S., Okou, O. C., Tuo, K., Kamenan, K. C. D., Gnondjui, A. A., Kouakou, L., Atteméné, D. S. D., Penali, L., Jambou, R. & Djaman, A. J. (2017). Propagation in culture of Plasmodium falciparum gametocytes clinical isolates and antiplasmodial activity of two medicinal plant extracts on parasitic growth. African Journal of Parasitology Research 4, 249-256.
[25]	Nassirou, R. S., Ibrahim, M. L., Ilagouma, A. T., Mahamadou, A., Mamoudou, M., Abdoulaye, A., Oukem-Boyer, O. O. M. & K, K. I. (2015). In vitro evaluation of the antiplasmodial activity of plant extracts from the traditional pharmacy.

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APA Style

Bohui, G. S., Beourou, S., Sanogo, D., N’guessan, J. D., Attioua, K. B., et al. (2024). Phytochemical Screening and Evaluation of the Antioxidant and Antiplasmodial Activities of "Ahoutou", a Recipe Used in the Treatment of Malaria in Ivory Coast. Science Journal of Chemistry, 12(5), 109-116. https://doi.org/10.11648/j.sjc.20241205.14

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ACS Style

Bohui, G. S.; Beourou, S.; Sanogo, D.; N’guessan, J. D.; Attioua, K. B., et al. Phytochemical Screening and Evaluation of the Antioxidant and Antiplasmodial Activities of "Ahoutou", a Recipe Used in the Treatment of Malaria in Ivory Coast. Sci. J. Chem. 2024, 12(5), 109-116. doi: 10.11648/j.sjc.20241205.14

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AMA Style

Bohui GS, Beourou S, Sanogo D, N’guessan JD, Attioua KB, et al. Phytochemical Screening and Evaluation of the Antioxidant and Antiplasmodial Activities of "Ahoutou", a Recipe Used in the Treatment of Malaria in Ivory Coast. Sci J Chem. 2024;12(5):109-116. doi: 10.11648/j.sjc.20241205.14

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@article{10.11648/j.sjc.20241205.14,
  author = {Gouegoui Serge-Pacome Bohui and Sylvain Beourou and Djibiliour Sanogo and Jean David N’guessan and Koffi Barthelemy Attioua and Augustin Amissa Adima},
  title = {Phytochemical Screening and Evaluation of the Antioxidant and Antiplasmodial Activities of "Ahoutou", a Recipe Used in the Treatment of Malaria in Ivory Coast
},
  journal = {Science Journal of Chemistry},
  volume = {12},
  number = {5},
  pages = {109-116},
  doi = {10.11648/j.sjc.20241205.14},
  url = {https://doi.org/10.11648/j.sjc.20241205.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20241205.14},
  abstract = {Recipes based on medicinal plants are frequently used to treat malaria in Ivory Coast. Among these recipes is Ahoutou, made from a mixture of the leaves of three plants, one of the most widely used in the village of Djahakro in the commune of Yamoussoukro. However, the use of medicinal plant-based recipes in the fight against malaria must be based on scientific evidence of safety, efficacy and quality. The aim of this study is to provide a scientific, phytochemical and pharmacological basis for the traditional therapeutic use of Ahoutou. To this end, a phytochemical study of the Ahoutou extract was carried out, the antioxidant activity was assessed using the DPPH test and then the in vitro anti-plasmodial activity was carried out on clinical isolates and on the reference strain Dd2. The phytochemical study revealed the presence of polyphenols, flavonoids, alkaloids, tannins, saponins, steroids and triterpenes. The antioxidant activity test showed that Ahoutou was capable of reducing the DPPH free radical. Ahoutou showed promising anti-plasmodial activity (IC50 =12.86 µg/mL) in the clinical strain and moderate activity (IC50 =23.40 µg/mL) in the reference strain. Our results would justify the use of this recipe in the treatment of malaria. We plan to study the toxicity of Ahoutou.
},
 year = {2024}
}

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TY  - JOUR
T1  - Phytochemical Screening and Evaluation of the Antioxidant and Antiplasmodial Activities of "Ahoutou", a Recipe Used in the Treatment of Malaria in Ivory Coast

AU  - Gouegoui Serge-Pacome Bohui
AU  - Sylvain Beourou
AU  - Djibiliour Sanogo
AU  - Jean David N’guessan
AU  - Koffi Barthelemy Attioua
AU  - Augustin Amissa Adima
Y1  - 2024/09/26
PY  - 2024
N1  - https://doi.org/10.11648/j.sjc.20241205.14
DO  - 10.11648/j.sjc.20241205.14
T2  - Science Journal of Chemistry
JF  - Science Journal of Chemistry
JO  - Science Journal of Chemistry
SP  - 109
EP  - 116
PB  - Science Publishing Group
SN  - 2330-099X
UR  - https://doi.org/10.11648/j.sjc.20241205.14
AB  - Recipes based on medicinal plants are frequently used to treat malaria in Ivory Coast. Among these recipes is Ahoutou, made from a mixture of the leaves of three plants, one of the most widely used in the village of Djahakro in the commune of Yamoussoukro. However, the use of medicinal plant-based recipes in the fight against malaria must be based on scientific evidence of safety, efficacy and quality. The aim of this study is to provide a scientific, phytochemical and pharmacological basis for the traditional therapeutic use of Ahoutou. To this end, a phytochemical study of the Ahoutou extract was carried out, the antioxidant activity was assessed using the DPPH test and then the in vitro anti-plasmodial activity was carried out on clinical isolates and on the reference strain Dd2. The phytochemical study revealed the presence of polyphenols, flavonoids, alkaloids, tannins, saponins, steroids and triterpenes. The antioxidant activity test showed that Ahoutou was capable of reducing the DPPH free radical. Ahoutou showed promising anti-plasmodial activity (IC50 =12.86 µg/mL) in the clinical strain and moderate activity (IC50 =23.40 µg/mL) in the reference strain. Our results would justify the use of this recipe in the treatment of malaria. We plan to study the toxicity of Ahoutou.

VL  - 12
IS  - 5
ER  -

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Author Information

Gouegoui Serge-Pacome Bohui

Department of Physics Chemistry, Felix Houphouet-Boigny University, Abidjan, Ivory Coast

Contact Email

http://orcid.org/0009-0007-9349-6920
Sylvain Beourou

Department of Parasitology-Mycology and Malariology Unit, Pasteur Institute in Ivory Coast, Abidjan, Ivory Coast

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Djibiliour Sanogo

Department of Physics Chemistry, Felix Houphouet-Boigny University, Abidjan, Ivory Coast

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Jean David N’guessan

Department of Biochemistry and Pharmacodynamics, Felix Houphouët-Boigny University, Abidjan, Ivory Coast

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Koffi Barthelemy Attioua

Department of Physics Chemistry, Felix Houphouet-Boigny University, Abidjan, Ivory Coast

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Augustin Amissa Adima

Department of Agro-Food Chemical Engineering, National Polytechnic Institute Felix Houphouet-Boigny, Yamoussoukro, Ivory Coast

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Plain Text BibTeX RIS

APA Style

Bohui, G. S., Beourou, S., Sanogo, D., N’guessan, J. D., Attioua, K. B., et al. (2024). Phytochemical Screening and Evaluation of the Antioxidant and Antiplasmodial Activities of "Ahoutou", a Recipe Used in the Treatment of Malaria in Ivory Coast. Science Journal of Chemistry, 12(5), 109-116. https://doi.org/10.11648/j.sjc.20241205.14

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ACS Style

Bohui, G. S.; Beourou, S.; Sanogo, D.; N’guessan, J. D.; Attioua, K. B., et al. Phytochemical Screening and Evaluation of the Antioxidant and Antiplasmodial Activities of "Ahoutou", a Recipe Used in the Treatment of Malaria in Ivory Coast. Sci. J. Chem. 2024, 12(5), 109-116. doi: 10.11648/j.sjc.20241205.14

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AMA Style

Bohui GS, Beourou S, Sanogo D, N’guessan JD, Attioua KB, et al. Phytochemical Screening and Evaluation of the Antioxidant and Antiplasmodial Activities of "Ahoutou", a Recipe Used in the Treatment of Malaria in Ivory Coast. Sci J Chem. 2024;12(5):109-116. doi: 10.11648/j.sjc.20241205.14

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@article{10.11648/j.sjc.20241205.14,
  author = {Gouegoui Serge-Pacome Bohui and Sylvain Beourou and Djibiliour Sanogo and Jean David N’guessan and Koffi Barthelemy Attioua and Augustin Amissa Adima},
  title = {Phytochemical Screening and Evaluation of the Antioxidant and Antiplasmodial Activities of "Ahoutou", a Recipe Used in the Treatment of Malaria in Ivory Coast
},
  journal = {Science Journal of Chemistry},
  volume = {12},
  number = {5},
  pages = {109-116},
  doi = {10.11648/j.sjc.20241205.14},
  url = {https://doi.org/10.11648/j.sjc.20241205.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20241205.14},
  abstract = {Recipes based on medicinal plants are frequently used to treat malaria in Ivory Coast. Among these recipes is Ahoutou, made from a mixture of the leaves of three plants, one of the most widely used in the village of Djahakro in the commune of Yamoussoukro. However, the use of medicinal plant-based recipes in the fight against malaria must be based on scientific evidence of safety, efficacy and quality. The aim of this study is to provide a scientific, phytochemical and pharmacological basis for the traditional therapeutic use of Ahoutou. To this end, a phytochemical study of the Ahoutou extract was carried out, the antioxidant activity was assessed using the DPPH test and then the in vitro anti-plasmodial activity was carried out on clinical isolates and on the reference strain Dd2. The phytochemical study revealed the presence of polyphenols, flavonoids, alkaloids, tannins, saponins, steroids and triterpenes. The antioxidant activity test showed that Ahoutou was capable of reducing the DPPH free radical. Ahoutou showed promising anti-plasmodial activity (IC50 =12.86 µg/mL) in the clinical strain and moderate activity (IC50 =23.40 µg/mL) in the reference strain. Our results would justify the use of this recipe in the treatment of malaria. We plan to study the toxicity of Ahoutou.
},
 year = {2024}
}

Copy | Download

TY  - JOUR
T1  - Phytochemical Screening and Evaluation of the Antioxidant and Antiplasmodial Activities of "Ahoutou", a Recipe Used in the Treatment of Malaria in Ivory Coast

AU  - Gouegoui Serge-Pacome Bohui
AU  - Sylvain Beourou
AU  - Djibiliour Sanogo
AU  - Jean David N’guessan
AU  - Koffi Barthelemy Attioua
AU  - Augustin Amissa Adima
Y1  - 2024/09/26
PY  - 2024
N1  - https://doi.org/10.11648/j.sjc.20241205.14
DO  - 10.11648/j.sjc.20241205.14
T2  - Science Journal of Chemistry
JF  - Science Journal of Chemistry
JO  - Science Journal of Chemistry
SP  - 109
EP  - 116
PB  - Science Publishing Group
SN  - 2330-099X
UR  - https://doi.org/10.11648/j.sjc.20241205.14
AB  - Recipes based on medicinal plants are frequently used to treat malaria in Ivory Coast. Among these recipes is Ahoutou, made from a mixture of the leaves of three plants, one of the most widely used in the village of Djahakro in the commune of Yamoussoukro. However, the use of medicinal plant-based recipes in the fight against malaria must be based on scientific evidence of safety, efficacy and quality. The aim of this study is to provide a scientific, phytochemical and pharmacological basis for the traditional therapeutic use of Ahoutou. To this end, a phytochemical study of the Ahoutou extract was carried out, the antioxidant activity was assessed using the DPPH test and then the in vitro anti-plasmodial activity was carried out on clinical isolates and on the reference strain Dd2. The phytochemical study revealed the presence of polyphenols, flavonoids, alkaloids, tannins, saponins, steroids and triterpenes. The antioxidant activity test showed that Ahoutou was capable of reducing the DPPH free radical. Ahoutou showed promising anti-plasmodial activity (IC50 =12.86 µg/mL) in the clinical strain and moderate activity (IC50 =23.40 µg/mL) in the reference strain. Our results would justify the use of this recipe in the treatment of malaria. We plan to study the toxicity of Ahoutou.

VL  - 12
IS  - 5
ER  -

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