Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats

Pozilov Mamurjon Komiljonovich; Ernazarov Zafar Mamurovich; Afzalova Sayyora Abdulahadovna; Asrarov Muzaffar Islamovich; Ergashev Nurali Azamovich; Komilov Baxrom Jamoldinovich

doi:doi:10.11648/j.ajbls.20200806.15

Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats

Pozilov Mamurjon Komiljonovich, Ernazarov Zafar Mamurovich, Afzalova Sayyora Abdulahadovna, Asrarov Muzaffar Islamovich, Ergashev Nurali Azamovich, Komilov Baxrom Jamoldinovich

Published in American Journal of Biomedical and Life Sciences (Volume 8, Issue 6)

Received: 11 November 2020 Accepted: 24 November 2020 Published: 4 December 2020

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Abstract

The state of the mitochondrial megapore (mitochondrial permeability transition pore-mPTP), respiration and oxidative phosphorylation of rat liver and pancreas mitochondria in streptozotocin (STZ) - induced diabetes were studied, considered the ways of correction of the detected membrane damage with the flavone luteolin isolated from the plant Inula caspica. It was shown that, under conditions of experimental diabetes mellitus, the rate of swelling of rat liver and pancreas mitochondria is higher than of the healthy ones; this means that mPTP of rat liver and pancreas mitochondria is in the open state in pathology. Luteolin recovers mPTP to the normal condition, thus removing the effect of STZ on mitochondria. It was also shown that, the respiration rate of liver and pancreatic mitochondria in the state 3 and state 4 states increases in STZ - induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The data obtained indicate the disconnection of respiration and oxidative phosphorylation in STZ - induced diabetes. Luteolin (oral dose is 50 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver and pancreas mitochondria, probably due to its antioxidant properties.

Published in	American Journal of Biomedical and Life Sciences (Volume 8, Issue 6)
DOI	10.11648/j.ajbls.20200806.15
Page(s)	220-224
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Liver, Pancreas, Mitochondria, mPTP, Lipid Peroxidation, Streptozotocin-induced Diabetes, Luteolin, Oxidative Phosphorylation

References

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[3]	Asrarov M. I., Pozilov M. K., Rahmatullaeva M. M. and Ergaschev N. A. (2014). The influence of the hypoglycemic agent glycorazmulin on the functional state of mitochondria in the rats with streptozotocin-induced diabetes, Problems of Endocrinology 60 (3): 38–42.
[4]	Newsholme P., Cruzat V F., Keane K N., Carlessi R. and Homem de Bittencourt P. I. (2016). Molecular mechanisms of ROS production and oxidative stress in diabetes, Biochem J, 473 (24): 4527-4550.
[5]	Roy J., Galano J. M., Durand T., Le Guennec J. Y. and Chung‐Yung Lee J. (2017). Physiological role of reactive oxygen species as promoters of natural defenses, The Faseb Journal, 31 (9): 3729-3745.
[6]	Zhao R. Z., Jiang S., Zhang L. and Yu Z. B. (2019). Mitochondrial electron transport chain, ROS generation and uncoupling, Int J Mol Med, 44 (1): 3-15.
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[8]	Newsholme P., Keane K. N., Carlessi R. and Cruzat V. (2019). Oxidative stress pathways in pancreatic β-cells and insulin-sensitive cells and tissues: importance to cell metabolism, function, and dysfunction, Am J Physiol Cell Physiol, 317. (3): 420-433.
[9]	Schneider W. C., Hageboom G. H. and Pallade G. E. (1948). Cytochemical studies of mammalian tissues; isolation of intact mitochondria from rat liver; some biochemical properties of mitochondria and submicroscopic particulate material, J. Biol. Chem, 172 (2): 619–635.
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[13]	Peterson G. L. (1977). A simplification of the protein assay method of Lowry et al., which is more generally applicable, Analytical biochemistry, 83 (2): 346-356.
[14]	Pozilov M. K., Asrarov M. I., Urmanova G. U. and Eshbakova K. A. (2015) Protective effect of salvifolin on liver mitochondrial function in rats with experimental diabetes, European science review, 7-8: 3–7.
[15]	Asrarov M. I., Pozilov M. K. and Ergashev N. A. (2015) Effect of a hypoglycemic agent glycorazmulin on the mitochondrial permeability transition pore at the alloxan-induced diabetes, The congress Euromedica-Hanover. Modern Aspects of diagnostics, treatment and rehabilitation, 12–13.

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Pozilov Mamurjon Komiljonovich, Ernazarov Zafar Mamurovich, Afzalova Sayyora Abdulahadovna, Asrarov Muzaffar Islamovich, Ergashev Nurali Azamovich, et al. (2020). Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats. American Journal of Biomedical and Life Sciences, 8(6), 220-224. https://doi.org/10.11648/j.ajbls.20200806.15

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ACS Style

Pozilov Mamurjon Komiljonovich; Ernazarov Zafar Mamurovich; Afzalova Sayyora Abdulahadovna; Asrarov Muzaffar Islamovich; Ergashev Nurali Azamovich, et al. Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats. Am. J. Biomed. Life Sci. 2020, 8(6), 220-224. doi: 10.11648/j.ajbls.20200806.15

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AMA Style

Pozilov Mamurjon Komiljonovich, Ernazarov Zafar Mamurovich, Afzalova Sayyora Abdulahadovna, Asrarov Muzaffar Islamovich, Ergashev Nurali Azamovich, et al. Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats. Am J Biomed Life Sci. 2020;8(6):220-224. doi: 10.11648/j.ajbls.20200806.15

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@article{10.11648/j.ajbls.20200806.15,
  author = {Pozilov Mamurjon Komiljonovich and Ernazarov Zafar Mamurovich and Afzalova Sayyora Abdulahadovna and Asrarov Muzaffar Islamovich and Ergashev Nurali Azamovich and Komilov Baxrom Jamoldinovich},
  title = {Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {8},
  number = {6},
  pages = {220-224},
  doi = {10.11648/j.ajbls.20200806.15},
  url = {https://doi.org/10.11648/j.ajbls.20200806.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20200806.15},
  abstract = {The state of the mitochondrial megapore (mitochondrial permeability transition pore-mPTP), respiration and oxidative phosphorylation of rat liver and pancreas mitochondria in streptozotocin (STZ) - induced diabetes were studied, considered the ways of correction of the detected membrane damage with the flavone luteolin isolated from the plant Inula caspica. It was shown that, under conditions of experimental diabetes mellitus, the rate of swelling of rat liver and pancreas mitochondria is higher than of the healthy ones; this means that mPTP of rat liver and pancreas mitochondria is in the open state in pathology. Luteolin recovers mPTP to the normal condition, thus removing the effect of STZ on mitochondria. It was also shown that, the respiration rate of liver and pancreatic mitochondria in the state 3 and state 4 states increases in STZ - induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The data obtained indicate the disconnection of respiration and oxidative phosphorylation in STZ - induced diabetes. Luteolin (oral dose is 50 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver and pancreas mitochondria, probably due to its antioxidant properties.},
 year = {2020}
}

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TY  - JOUR
T1  - Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats
AU  - Pozilov Mamurjon Komiljonovich
AU  - Ernazarov Zafar Mamurovich
AU  - Afzalova Sayyora Abdulahadovna
AU  - Asrarov Muzaffar Islamovich
AU  - Ergashev Nurali Azamovich
AU  - Komilov Baxrom Jamoldinovich
Y1  - 2020/12/04
PY  - 2020
N1  - https://doi.org/10.11648/j.ajbls.20200806.15
DO  - 10.11648/j.ajbls.20200806.15
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 220
EP  - 224
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20200806.15
AB  - The state of the mitochondrial megapore (mitochondrial permeability transition pore-mPTP), respiration and oxidative phosphorylation of rat liver and pancreas mitochondria in streptozotocin (STZ) - induced diabetes were studied, considered the ways of correction of the detected membrane damage with the flavone luteolin isolated from the plant Inula caspica. It was shown that, under conditions of experimental diabetes mellitus, the rate of swelling of rat liver and pancreas mitochondria is higher than of the healthy ones; this means that mPTP of rat liver and pancreas mitochondria is in the open state in pathology. Luteolin recovers mPTP to the normal condition, thus removing the effect of STZ on mitochondria. It was also shown that, the respiration rate of liver and pancreatic mitochondria in the state 3 and state 4 states increases in STZ - induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The data obtained indicate the disconnection of respiration and oxidative phosphorylation in STZ - induced diabetes. Luteolin (oral dose is 50 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver and pancreas mitochondria, probably due to its antioxidant properties.
VL  - 8
IS  - 6
ER  -

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Author Information

Pozilov Mamurjon Komiljonovich

Institute of Biophysics and Biochemistry at the National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
Ernazarov Zafar Mamurovich

Institute of Biophysics and Biochemistry at the National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
Afzalova Sayyora Abdulahadovna

Department of Human and Animal Physiology, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
Asrarov Muzaffar Islamovich

Institute of Biophysics and Biochemistry at the National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
Ergashev Nurali Azamovich

Institute of Biophysics and Biochemistry at the National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan
Komilov Baxrom Jamoldinovich

Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan

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APA Style

Pozilov Mamurjon Komiljonovich, Ernazarov Zafar Mamurovich, Afzalova Sayyora Abdulahadovna, Asrarov Muzaffar Islamovich, Ergashev Nurali Azamovich, et al. (2020). Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats. American Journal of Biomedical and Life Sciences, 8(6), 220-224. https://doi.org/10.11648/j.ajbls.20200806.15

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ACS Style

Pozilov Mamurjon Komiljonovich; Ernazarov Zafar Mamurovich; Afzalova Sayyora Abdulahadovna; Asrarov Muzaffar Islamovich; Ergashev Nurali Azamovich, et al. Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats. Am. J. Biomed. Life Sci. 2020, 8(6), 220-224. doi: 10.11648/j.ajbls.20200806.15

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AMA Style

Pozilov Mamurjon Komiljonovich, Ernazarov Zafar Mamurovich, Afzalova Sayyora Abdulahadovna, Asrarov Muzaffar Islamovich, Ergashev Nurali Azamovich, et al. Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats. Am J Biomed Life Sci. 2020;8(6):220-224. doi: 10.11648/j.ajbls.20200806.15

Copy | Download

@article{10.11648/j.ajbls.20200806.15,
  author = {Pozilov Mamurjon Komiljonovich and Ernazarov Zafar Mamurovich and Afzalova Sayyora Abdulahadovna and Asrarov Muzaffar Islamovich and Ergashev Nurali Azamovich and Komilov Baxrom Jamoldinovich},
  title = {Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {8},
  number = {6},
  pages = {220-224},
  doi = {10.11648/j.ajbls.20200806.15},
  url = {https://doi.org/10.11648/j.ajbls.20200806.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20200806.15},
  abstract = {The state of the mitochondrial megapore (mitochondrial permeability transition pore-mPTP), respiration and oxidative phosphorylation of rat liver and pancreas mitochondria in streptozotocin (STZ) - induced diabetes were studied, considered the ways of correction of the detected membrane damage with the flavone luteolin isolated from the plant Inula caspica. It was shown that, under conditions of experimental diabetes mellitus, the rate of swelling of rat liver and pancreas mitochondria is higher than of the healthy ones; this means that mPTP of rat liver and pancreas mitochondria is in the open state in pathology. Luteolin recovers mPTP to the normal condition, thus removing the effect of STZ on mitochondria. It was also shown that, the respiration rate of liver and pancreatic mitochondria in the state 3 and state 4 states increases in STZ - induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The data obtained indicate the disconnection of respiration and oxidative phosphorylation in STZ - induced diabetes. Luteolin (oral dose is 50 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver and pancreas mitochondria, probably due to its antioxidant properties.},
 year = {2020}
}

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TY  - JOUR
T1  - Effect of the Plant Flavonoid Luteolin on a Mitochondrial Function in the Streptozotocin-induced Diabetic Rats
AU  - Pozilov Mamurjon Komiljonovich
AU  - Ernazarov Zafar Mamurovich
AU  - Afzalova Sayyora Abdulahadovna
AU  - Asrarov Muzaffar Islamovich
AU  - Ergashev Nurali Azamovich
AU  - Komilov Baxrom Jamoldinovich
Y1  - 2020/12/04
PY  - 2020
N1  - https://doi.org/10.11648/j.ajbls.20200806.15
DO  - 10.11648/j.ajbls.20200806.15
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 220
EP  - 224
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20200806.15
AB  - The state of the mitochondrial megapore (mitochondrial permeability transition pore-mPTP), respiration and oxidative phosphorylation of rat liver and pancreas mitochondria in streptozotocin (STZ) - induced diabetes were studied, considered the ways of correction of the detected membrane damage with the flavone luteolin isolated from the plant Inula caspica. It was shown that, under conditions of experimental diabetes mellitus, the rate of swelling of rat liver and pancreas mitochondria is higher than of the healthy ones; this means that mPTP of rat liver and pancreas mitochondria is in the open state in pathology. Luteolin recovers mPTP to the normal condition, thus removing the effect of STZ on mitochondria. It was also shown that, the respiration rate of liver and pancreatic mitochondria in the state 3 and state 4 states increases in STZ - induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The data obtained indicate the disconnection of respiration and oxidative phosphorylation in STZ - induced diabetes. Luteolin (oral dose is 50 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver and pancreas mitochondria, probably due to its antioxidant properties.
VL  - 8
IS  - 6
ER  -

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